Tuesday's 2.3 magnitude quake struck around Boronia and Ferntree Gully just after 5pm, at a depth of about 4km.
More than 140 residents reported feeling the quake, according to Geoscience Australia.
It comes days after a 4.0-magnitude quake struck 3km deep in north-west Melbourne on Sunday.
Many people were woken by tremors, with reports it was felt as far from Sunbury to Hobart and an SRC site in Adelaide.
In 2021 a 5.9-magnitude quake hit near Mansfield, 180km north-east of Melbourne, with tremors lasting for between 30 and 40 seconds.
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